Bcl-2核酶诱导SMMC7721细胞凋亡与p16,p21蛋白表达的关系研究

Bcl-2核酶诱导SMMC7721细胞凋亡与p16,p21蛋白表达的关系研究

减小字体 增大字体 作者:佚名  来源:本站整理  发布时间:2009-06-29 23:17:19

摘要:目的 观察bcl-2核酶对人肝癌细胞株SMMC-7721细胞的作用,并检测p16和p21的表达情况。 探讨bcl-2核酶在肝癌治疗中的意义。方法 经脂质体介导的方法,将PMTr-neo(正向bcl-2核 酶真核表达载体)导入SMMC7721细胞中。细胞克隆转移扩大培养后,采用TUNEL法、免疫组化技术结 合图像分析,在检测SMMC7721/PMTr-neo细胞凋亡的同时,检测p16, p21的表达。结果与对照组相比较 ,在 bcl-2核酶诱发SMMC7721细胞发生凋亡的同时,p16和p21基因的表达水平显著增高。 结论 bcl-2核酶通过封闭bcl-2的表达可促进SMMC7721细胞凋亡,同时伴发p21和p16表达水平的增高。


 

Relationship between apoptosis induced with bcl-2 ribozyme and expression of p16, p21 in SMMC7721 cells


  Abstract: Aim To observe the effect of bcl-2 ribozyme on SMMC7721 cells and the expression of p16, p21,so to investigate the role of bcl-2 ribozyme in hepatocarcinoma treatment. Methods PMTr neo (sense bcl-2 ribozyme eucaryotic expression vector) was introduced into SMMC7221 cells through lipofectin mediation. After cloning, TUNEL and IHC in combination with imaging analysis were used to simultaneously determine apoptosis and p16, p21 expressions of the SMMC7721/PMTr neo cells. Results Compared with control, in the course of apoptosis induced by bcl-2 ribozyme p16 and p21 expressions rose significantly in SMMC7721 cells. Conclusion bcl-2 ribozyme can promote SMMC7721 cell apoptosis by blocking bcl-2 expression, which is accompanied by an increased p16 and p21 expressions. This result could be of value for studying hepatocarcinoma genesis and probing therapeutic procedure. neo (sense bcl-2 ribozyme eucaryotic expression vector) was introduced into SMMC7221 cells through lipofectin mediation. After cloning, TUNEL and IHC in combination with imaging analysis were used to simultaneously determine apoptosis and p16, p21 expressions of the SMMC7721/PMTr neo cells. Results Compared with control, in the course of apoptosis induced by bcl-2 ribozyme p16 and p21 expressions rose significantly in SMMC7721 cells. Conclusion bcl-2 ribozyme can promote SMMC7721 cell apoptosis by blocking bcl-2 expression, which is accompanied by an increased p16 and p21 expressions. This result could be of value for studying hepatocarcinoma genesis and probing therapeutic procedure.

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